桉树叶二种酶和蛋白质的季节与年际变化

doi:10.14088/j.cnki.issn0439-8114.2018.10.017

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (10): 73-76.doi: 10.14088/j.cnki.issn0439-8114.2018.10.017

桉树叶二种酶和蛋白质的季节与年际变化

朱宇林, 周兴文, 黄丽玲, 苏建睦, 赵英

玉林师范学院生物与制药学院,广西玉林 537000

收稿日期:2017-12-15 出版日期:2018-05-25 发布日期:2019-12-19
作者简介:朱宇林（1975-）,男,广西玉林人,教授,博士,主要从事林木生理生态学和森林培育学等方面的教学与研究,（电话）13877525713（电子信箱）gxzyl@163.com
基金资助:
广西自然科学基金项目（2014GXNSFAA118083）; 广西大学生创新创业训练计划项目（201510606070）

Dynamic Changes of Two Kinds of Enzymes and Soluble Protein of Eucalyptus Leaves in Different Seasons and Years

ZHU Yu-lin, ZHOU Xing-wen, HUANG Li-ling, SU Jian-mu, ZHAO Ying

College of Biology, Pharmacy of Yulin Normal University, Yulin537000, Guangxi, China

Received:2017-12-15 Online:2018-05-25 Published:2019-12-19

摘要/Abstract

摘要： 桉树是中国南方地区的重要用材树种,探讨其贮藏氮素循环机制对于提高桉树人工林氮素利用水平具有一定的研究意义。试验以桉树枝叶组织为研究对象,在观察其物候期基础上,采用紫外分光度法测定了桉树叶蛋白质含量及谷氨酸合成酶和谷氨酰胺合成酶活性的动态变化。结果表明,秋季桉树叶中的可溶性蛋白含量最低,而在次年的春季其含量最高;夏季其含量则处在春季和秋季中间。谷氨酸合成酶活性在秋季为最高,在次年春季为最低。谷氨酰胺合成酶活性在秋季表现为最高,在次年的春季则表现为最低。桉树叶中的可溶性蛋白含量、谷氨酸合成酶活性以及谷氨酰胺合成酶活性与年份和季节密切相关,三者分别呈现出不同的变化趋势。

关键词: 桉树, 可溶性蛋白, 谷氨酰胺合成酶, 谷氨酸合成酶

Abstract: Eucalyptus is an important economic tree species in southern China. Research on storage metabolism of eucalyptus would increase its nitrogen utilization level, which had certain research significance. Taking the branch and leaf tissue of eucalyptus as research object, the dynamic change of protein content as well as the activity of glutamate synthetase and glutamine synthetase was observed using UV spectrophotometric method based on the phonological phase. The results showed that, in the beginning of autumn, soluble protein content of eucalyptus leaves was at the lowest level; But in the following spring it was the highest. The soluble protein content in summer was between spring and the autumn. Glutamate synthese activity was the highest in autumn and the lowest in spring. The glutamine synthetase activity was the highest in autumn while lowest in spring. The glutamate synthetase activity, soluble protein content, glutamine synthetase activity was closely related to the season and year, the three of which present different changing trends.

Key words: eucalyptus, soluble protein, glutamate synthetase, glutamine synthetase

中图分类号:

S792.39

朱宇林, 周兴文, 黄丽玲, 苏建睦, 赵英. 桉树叶二种酶和蛋白质的季节与年际变化[J]. 湖北农业科学, 2018, 57(10): 73-76.

ZHU Yu-lin, ZHOU Xing-wen, HUANG Li-ling, SU Jian-mu, ZHAO Ying. Dynamic Changes of Two Kinds of Enzymes and Soluble Protein of Eucalyptus Leaves in Different Seasons and Years[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(10): 73-76.

参考文献

[1] KUSANO M,TABUCHI M,FUKUSHIMA A,et al.Metabolomics data reveal a crucial role of cytosolic glutamine synthetase 1;1 in coordinating metabolic balance in rice[J].Plant J,2011,66(3):456-466.
[2] 冯万军,邢国芳,牛旭龙,等.植物谷氨酰胺合成酶研究进展及其应用前景[J].生物工程学报,2015,31(9):1301-1312.
[3] 张华,田维敏.树木营养器官贮藏氮化物性质的研究进展[J].华南热带农业大学学报,2007,13(2):37-40.
[4] 朱宇林,何斌,杨钙仁,等.尾巨桉人工林营养元素积累及其生物循环特征[J].东北林业大学学报,2012,40(6):8-11,66.
[5] 韩艺师,魏彦昌,欧阳志云,等.连栽措施对桉树人工林结构及持水性能的影响[J].生态学报,2008,28(9):4609-4617.
[6] 何斌,黄承标,秦武明,等.不同植被恢复类型对土壤性质和水源涵养功能的影响[J].水土保持学报,2009,23(2):71-75.
[7] 陈慧洁,郭朦朦,冯丽贞,等.桉树叶片双向电泳体系的建立及优化[J].福建农林大学学报(自然科学版),2015,44(1):46-50.
[8] 马松亚,蒋维昕,屈林丰,等.桉树无性系叶片蛋白质组双向电泳体系优化[J].中南林业科技大学学报,2016,36(4):45-50.
[9] 李小方,张志良.植物生理学实验指导[M].第五版.北京:高等教育出版社,2016.
[10] 熊丹. 水稻灌浆期弱光对籽粒氨基酸积累及相关酶活性的影响[D].成都:四川农业大学,2012.
[11] 金正勋,钱春荣,杨静,等.水稻灌浆成熟期籽粒谷氨酰胺合成酶活性变化及其与稻米品质关系的初步研究[J].中国水稻科学,2007,21(1):103-106.
[12] 黄绍辉,刘艳,王静静,等.冬春季节气温变化与樟树叶片可溶性蛋白质含量的关联分析[J].徐州工程学院学报(自然科学版),2015(4):61-65.
[13] 翁伯琦,郑向丽,赵婷,等.不同生育期花生叶片蛋白质含量及氮代谢相关酶活性分析[J].植物资源与环境学报,2014, 23(1):65-70.
[14] 余佳玲,朱兆坤,张振华,等.不同供氮条件下谷氨酰胺合成酶与谷氨酸合成酶对油菜氮素再利用的影响[J].作物杂志,2014(6):81-85.
[15] MIFLIN B J,HABASH D Z.The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities form provement in the nitrogen utilization of crops[J].Journal of Experimental Botany,2002,53(2):979-987.
[16] 刚爽,王敬国,杨亮,等.氮素用量对寒地水稻氮代谢关键酶活性的影响[J].农业现代化研究,2010,31(2):224-227.
[17] MIFLIN B J,LEA P J.Ammonia Assimilation in the Biochemistry of Plants,Amino Acids and Their Derivatives[M].New York:Academic Press,1980.169-202.

桉树叶二种酶和蛋白质的季节与年际变化

Dynamic Changes of Two Kinds of Enzymes and Soluble Protein of Eucalyptus Leaves in Different Seasons and Years

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	陈真真, 周国勤, 陈新宏, 谢旭东, 陈金平. 转华山新麦草果聚糖合成酶基因对烟草相关生理指标的影响[J]. 湖北农业科学, 2020, 59(9): 95-98.
[2]	莫小锋, 邓戴云, 文清岚, 黄文全, 陈江平. 草甘膦的不同处理对桉树伐桩催腐的影响[J]. 湖北农业科学, 2019, 58(24): 133-135.
[3]	李萃邦, 徐胜臻, 徐文兴, 曹敏惠. 桉树叶活性成分提取方法及生物活性研究进展[J]. 湖北农业科学, 2018, 57(6): 12-16.