海绵共附生真菌FWZ0415代谢产物的研究

doi:10.14088/j.cnki.issn0439-8114.2018.22.018

湖北农业科学 ›› 2018, Vol. 57 ›› Issue (22): 64-66.doi: 10.14088/j.cnki.issn0439-8114.2018.22.018

海绵共附生真菌FWZ0415代谢产物的研究

钱纯果¹, 周义权², 杨曦亮¹, 李奎³, 石丽桥³

1.武汉科技大学医学院药学系,武汉 430065;
2.仙桃市农业局,湖北仙桃 433000;
3.湖北省生物农药工程研究中心,武汉 430064

收稿日期:2018-08-29 出版日期:2018-11-25 发布日期:2019-12-19
通讯作者: 李奎,男,助理研究员,主要从事植物保护研究,（电话）13720238353（电子邮箱）likui1027@163.com。
作者简介:钱纯果（1997-）,女,湖北枣阳人,在读本科生,（电话）15871811923;石丽桥,女,副研究员,主要从事病原生物学研究,（电子邮箱）liqiao.shi@nberc.com
基金资助:
湖北省农业科技创新中心项目（2016-620-000-001-039）; 湖北省青年科学基金项目（2018NKYJJ21）

Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415

QIAN Chun-guo¹, ZHOU Yi-quan², YANG Xi-liang¹, LI Kui³, SHI Li-qiao³

1.Department of Pharmacy,School of Medicine,Wuhan University of Science and Technology,Wuhan 430065,China;
2.Xiantao Agricultural Bureau,Xiantao 433000,Hubei,China;
3.Hubei Biopesticide Engineering Research Center,Wuhan 430064,China

Received:2018-08-29 Online:2018-11-25 Published:2019-12-19

摘要/Abstract

摘要： 对海绵共附生真菌FWZ0415进行固体发酵培养,并对其发酵培养物的次级代谢产物进行分离纯化和结构鉴定等方面的研究。运用常压硅胶柱层析、葡聚糖凝胶柱（Sephadex LH-20）和半制备高效液相色谱（HPLC）等手段,从该菌株固体发酵培养物的提取物中分离纯化得到两个化合物,并运用核磁共振¹H NMR、¹³C NMR等手段鉴定这两个化合物的结构为violaceol-I和violaceol-II。测试这两个化合物对肿瘤细胞系K252、MCF-7、SGC7901的细胞毒性,violaceol-I未见显著的细胞毒性（IC₅₀>30 μmol）,violaceol-II对3株肿瘤细胞系的IC₅₀分别为16.7、16.3、15.8 μmol。

关键词: 海绵共附生真菌, 次生代谢产物, 分离纯化, 结构鉴定, 抗肿瘤活性

Abstract: Sponge epiphytic fungi FWZ0415 was cultured in solid state fermentation,and its secondary metabolites were isolated,purified and identified. Using silica gel column chromatography,Sephadex column（Sephadex LH-20） and the preparation of semi preparative high performance liquid chromatography（HPLC） methodtwo compounds were purified,and then identified as violaceol-I and violaceol-II by nuclear magnetic resonance 1H-NMR and 13C-NMR. The cytotoxicity of two compounds to K252,MCF-7 and SGC7901 were tested,violaceol-I was not significantly cytotoxicity（IC₅₀>30 μmol）. The IC₅₀ of violaceol-II to three tumor cell lines were 16.7,16.3,15.8 μmol,respectively.

Key words: spongye epiphytic fungi, secondary metabolites, isolation and purification, structural identification, antitumor activity

中图分类号:

R284
R285

钱纯果, 周义权, 杨曦亮, 李奎, 石丽桥. 海绵共附生真菌FWZ0415代谢产物的研究[J]. 湖北农业科学, 2018, 57(22): 64-66.

QIAN Chun-guo, ZHOU Yi-quan, YANG Xi-liang, LI Kui, SHI Li-qiao. Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(22): 64-66.

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海绵共附生真菌FWZ0415代谢产物的研究

Study on Secondary Metabolity from Spongye Epiphytic Fungi FWZ0415

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