湖北农业科学 ›› 2018, Vol. 57 ›› Issue (14): 99-101.doi: 10.14088/j.cnki.issn0439-8114.2018.14.023

• 动物科学 • 上一篇    下一篇

五株鸡滑液囊支原体的分离与鉴定

马爽, 于国营, 范根成, 郭莉莉, 赵鹏, 宫晓, 宋新宇   

  1. 青岛易邦生物工程有限公司/动物基因工程疫苗国家重点实验室, 山东 青岛 266114
  • 收稿日期:2018-02-06 出版日期:2018-07-25 发布日期:2019-12-19
  • 作者简介:马 爽(1982-), 女, 辽宁绥中人, 兽医师, 主要从事兽用生物制品研发工作, (电话)13953202845(电子信箱)yebiomashuang@126.com。

Isolation and Identification of Five Strains of Mycoplasma synoviae

MA Shuang, YU Guo-ying, FAN Gen-cheng, GUO Li-li, ZHAO Peng, GONG Xiao, SONG Xin-yu   

  1. Qingdao Yebio Bioengineering Co., Ltd./State Key Laboratory of Animal Genetic Engineering Vaccine, Qingdao 266114, Shandong, China
  • Received:2018-02-06 Online:2018-07-25 Published:2019-12-19

摘要: 从12份鸡滑液囊支原体临床病料中分离到5株支原体, 经形态特性、生化特性、培养特性、分子生物学特性鉴定, 5株支原体均为鸡滑液囊支原体。活菌计数结果表明, HN3株菌液的活菌数相对较高, 为109 CCU/mL;毒力试验结果表明, 5株鸡滑液囊支原体对45日龄SPF鸡的发病率为50%~90%, HN3株毒力相对最高, 为90%。

关键词: 鸡滑液囊支原体, 分离, 鉴定

Abstract: Five strains of Mycoplasma synoviae were isolated from twelve suspected samples with clinical symptoms of Mycoplasma synoviae infection.Morphologic observation, biochemical test and molecular biological analysis were used to identify these strains. The results showed that the five strains were all Mycoplasma synoviae. Live bacteria counting results showed that Strain HN3 had highest number of living bacterium, 109 CCU/mL. Virulence test results showed that morbidities of five strains to 45 days-old SPF chicken were 50%~90%. Strain HN3 had highest morbidity, was 90%.

Key words: Mycoplasma synoviae, isolation, identification

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