湖北农业科学 ›› 2018, Vol. 57 ›› Issue (23): 148-152.doi: 10.14088/j.cnki.issn0439-8114.2018.23.035

• 生物工程 • 上一篇    下一篇

湘鄂地区三叶木通野生资源的RAPD亲缘关系分析

李红英1, 王晓辉1, 覃大吉1, 向极钎1, 杨永康1, 陈菲菲1, 万海英1, 周敏2, 朱麟1   

  1. 1.恩施土家族苗族自治州农业科学院,湖北 恩施 445000;
    2.恩施市舞阳办事处农业技术中心,湖北 恩施 445000
  • 收稿日期:2018-08-29 出版日期:2018-12-10 发布日期:2019-12-18
  • 通讯作者: 覃大吉(1965-),男,湖北恩施人,正高职高级农艺师,主要从事作物遗传育种与栽培生理研究,(电话)13593603998(电子信箱)qindaji1127@163.com。
  • 作者简介:李红英(1982-),女,湖北利川人,助理研究员,在读硕士研究生,主要从事作物遗传与生物技术的研究工作,(电话)13872740626(电子信箱)522070555@qq.com
  • 基金资助:
    恩施州研究与开发计划项目“三叶木通野生转家种关键技术研究”

Analysis on the RAPD Phylogenetic Relationship of Akebia trifoliata(Thunb.) Koidz. in Hunan and Hubei Provinces

LI Hong-ying1, WANG Xiao-hui1, QIN Da-ji1, XIANG Ji-qian1, YANG Yong-kang1, CHENG Fei-fei1, WAN Hai-ying1, ZHOU Ming2, ZHU Lin1   

  1. 1.Enshi Autonomous Prefecture Academy of Agricultural Sciences,Enshi 445000,Hubei,China;
    2.Wuyangba Township Agricultural Technology Centre of Enshi City,Enshi 445000,Hubei,China
  • Received:2018-08-29 Online:2018-12-10 Published:2019-12-18

摘要: 采用RAPD分子标记技术对湘鄂地区6个不同区域的野生三叶木通[Akebia trifoliata(Thunb.) Koidz.]进行了遗传多样性研究。从50个随机引物中筛选出10个条带清晰、多态性明显并且重复性好的引物,共扩增出86条DNA片段,其中73条为多态性条带,占总扩增带数的84.9%。利用NTSYS软件进行聚类分析,以相似系数0.578为标准,可将所有品种分为3类,第Ⅰ大类包括恩施红庙(紫色型)和湖南古丈(麻色型);第Ⅱ类包括建始花坪、巴东茶店子和宣恩椒园;恩施太阳河的独立分为第Ⅲ类。

关键词: 三叶木通[Akebia trifoliata(Thunb.) Koidz.], 野生资源, RAPD, 亲缘关系

Abstract: The genetic diversity of Akebia trifoliata(Thunb.) Koidz. in six different areas of Hubei and Hunan provinces was studied by RAPD molecular marker. A total of 86 DNA fragments were screened out from 50 random primers with 10 clear bands,obvious polymorphism and good repeatability. Among them,73 were polymorphic bands,accounting for the total number of bands amplified of 84.9%. Cluster analysis using NTSYS software,the similarity coefficient of 0.578 as a standard,all varieties can be divided into three categories,the major category Ⅰ includes samples from Enshi Hongmiao(purple type) and Hunan Guzhang county(brown type); the category Ⅱ includes samples from Jianshi Huaping,Badong Chadianzi and Xuan’en Jiaoyuan;Samples from Enshi Taiyanghe is divided into a independent category.

Key words: Akebia trifoliata(Thunb.) Koidz., wild resources, RAPD, genetic relationship

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