桑树光合作用转录组研究

doi:10.14088/j.cnki.issn0439-8114.2019.23.023

湖北农业科学 ›› 2019, Vol. 58 ›› Issue (23): 101-111.doi: 10.14088/j.cnki.issn0439-8114.2019.23.023

桑树光合作用转录组研究

李勇^{1, 2}, 于翠², 莫荣利², 邓文², 熊超², 庄楚雄¹, 胡兴明²

1.华南农业大学生命科学学院,广州 510642;
2.湖北省农业科学院经济作物研究所,武汉 430064

收稿日期:2019-11-04 出版日期:2019-12-10 发布日期:2019-12-18
通讯作者: 庄楚雄,男,湖南岳阳人,研究员,主要从事生物化学与分子生物学研究工作,（电子信箱）zhuangcx@scau.edu.cn;胡兴明,男,湖南石门人,研究员,主要从事桑树育种与栽培研究工作,（电话）027-87526088 （电子信箱）13607121598@163.com。
作者简介:李勇（1980-）,男,山东菏泽人,助理研究员,博士研究生,主要从事桑树育种与栽培研究工作,（电话）027-87106003（电子信箱）liyong8057@163.com
基金资助:
国家现代农业产业技术体系建设专项（CARS-18-ZJ0208）

Research on mulberry photosynthesis transcriptome

LI Yong^{1, 2}, YU Cui², MO Rong-li², DENG Wen², XIONG Chao², ZHUANG Chu-xiong¹, HU Xing-ming²

1.College of Life Sciences,South China Agricultural University,Guangzhou 510642,China;
2.Economic Crops Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China

Received:2019-11-04 Online:2019-12-10 Published:2019-12-18

摘要/Abstract

摘要： 研究高产桑品种鄂桑1号（E1）和对照桑品种湖桑32号（H32）光合特性及光合相关基因表达变化,为桑树高光效育种以及种质资源筛选提供理论依据。利用LI-6400XT型便携式光合测定仪测定桑树叶片气体交换和叶绿素荧光参数,同时测定叶绿素含量和1,5二磷酸核酮糖羧化酶（RUBP）活性,在桑树光合日变化曲线峰值和谷底取样进行转录组测序,比较不同桑品种光合相关基因表达的差异。对E1和H32的光合日变化测定结果显示,桑品种E1叶片的Pn峰值显著大于H32峰值（P<0.05）,H32叶片Tr峰值显著大于E1峰值（P<0.05）;不同品种桑树叶片Pn-PAR和Pn-Ci的响应中,CE、CSP为E大于H32,AQY、LCP、LSP、CCP为H32大于E1。E1在较弱光强下实现光合产物积累的能力强于H32,E1对CO₂低浓度的利用效率优于H32;不同桑品种叶片叶绿素荧光参数中,E1叶片ΦPSⅡ和qP显著大于H32（P<0.05）,H32的NPQ显著大于E1（P<0.05）,表明H32叶片吸收的光能较多的以热能的形式进行耗散;H32的叶绿素a、叶绿素b和叶绿素含量均显著小于E1（P<0.05）,E1的RUBP活性大于H32（P>0.05）,表明E1光合能力优于H32。不同品种不同时段发现3 356个差异表达基因（Differentially expressed genes,DEGs）,代谢通路分析显示共有1 136个DEGs注释到KEEG数据库,DEGs显著富集的光合作用相关代谢通路主要是光合作用固碳、碳代谢、卟啉和叶绿素代谢等3条光合相关代谢通路。在FC≥2且FDR<0.01的高差异表达基因中筛选功能描述与光合作用相关的新基因共10个。其中Mulberry_newGene_3646、Mulberry_newGene_1405、Mulberry_newGene_2419和Mulberry_newGene_320共4个DEGs可能是造成H32和E1净光合速率和产量差异的关键基因,可重点研究。

关键词: 桑树（Morus alba L.）, 光合特性, 叶绿素荧光特性, 转录组

Abstract: The photosynthesis characteristics and photosynthetic related gene expression of high-yield mulberry varieties Esang 1 （E1） and control mulberry variety Husang 32（H32） were studied to provide a theoretical basis for high-efficiency breeding of mulberry trees and screening of germplasm resources. Using LI-6400XT mulberry leaf gas exchange and chlorophyll fluorescence parameters, chlorophyll content and ribulose carboxylase（RUBP） activity were measured simultaneously, transcriptome sequencing was performed on the peaks and valleys of mulberry photosynthesis curves, and the differences in photosynthetic related gene expression among different mulberry varieties were compared. The results of photosynthetic variation of E1 and H32 showed that the Pn peak of E1 leaves was significantly larger than that of H32（P<0.05）, and the peak of Tr of H32 leaves was significantly larger than that of E1 （P<0.05）. In the Pn-PAR and Pn-Ci responses of different mulberry leaves, CE and CSP were E1>H32, AQY, LCP, LSP and CCP were H32>E1. The ability of E1 to achieve photosynthetic product accumulation under weaker light intensity is stronger than that of H32, and the utilization efficiency of E1 to low concentration CO₂ is better than that of H32; among the chlorophyll fluorescence parameters of different mulberry varieties, ΦPSII and qP of E1 leaves were significantly higher than H32 （P<0.05）, and NPQ of H32 was significantly larger than E1 （P<0.05）, indicating that the light energy absorbed by H32 leaves was mainly dissipated in the form of heat energy; the chlorophyll a, chlorophyll b and chlorophyll content of H32 were significantly lower than E1（P<0.05）, and the RUBP activity of E1 was higher than that of H32（P>0.05）, indicating that the photosynthetic capacity of E1 was better than that of H32. 3 356 DEGs were found in different varieties and different time periods. The metabolic pathway analysis showed that there were 1 136 DEGs annotated to the KEEG database. The photosynthesis-related metabolic pathways significantly enriched by DEGs were mainly photosynthetic carbon sequestration, carbon metabolism, porphyrin and chlorophyll metabolism. Photosynthetically related metabolic pathways. A total of 10 new genes related to photosynthesis were screened for highly differentially expressed genes with FC≥2 and FDR<0.01. The four DEGs, Mulberry_newGene_3646, Mulberry_newGene_1405, Mulberry_newGene_2419 and Mulberry_newGene_320, may be the key genes causing the difference in net photosynthetic rate and yield between H32 and E1, which can be studied intensively.

Key words: mulberry, photosynthetic characteristics, chlorophyll fluorescence characteristics, transcriptome

中图分类号:

S888

李勇, 于翠, 莫荣利, 邓文, 熊超, 庄楚雄, 胡兴明. 桑树光合作用转录组研究[J]. 湖北农业科学, 2019, 58(23): 101-111.

LI Yong, YU Cui, MO Rong-li, DENG Wen, XIONG Chao, ZHUANG Chu-xiong, HU Xing-ming. Research on mulberry photosynthesis transcriptome[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(23): 101-111.

参考文献 29

[1]	许大全. 光合作用效率[M].上海:上海科学技术出版社,2002.
[2]	CHEN F G,CHEN L G,ZHAO H X,et al.Sex-specific responses and tolerances of Populus cathayana to salinity[J].Physiol plant 2010,140:163-173.
[3]	PENUELAS J,FILELLA I,LLUSIA J,et al.Comparative field study of spring and summer leaf gas exchange and photobiology of the Mediterranean trees Quercusilex and Phillyrea latifolia[J].Journal of experiment botany,1998,49(319):229-238.
[4]	BERRY J A,DOWNTON W J S. Environmental regulation of photosynthesis[A].Govindjee photosynthesis[M].New York:Academic Press,1982.
[5]	LONG S P,BAKER N R,RAINS C A.Analyzing the responses of photosynthetic CO2 assimilation to long-term elevation of atmospheric CO2 concentration[J].Vegetatio,1993,104:33-45.
[6]	GENTY B,BRIANTIS J M,BAKER N R.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochim Biophys Acta,1989,894:183-192.
[7]	DEMMIG B,WINTER K,KRUGER A,et al.Photoinhibition and zeaxanthin formation in intact leaves:A possible role of the xanthophyllcycle in the dissipation of excess light[J].Plant physiology,1987,84:218-224.
[8]	PORRA RJ,THOMPSON WA,KRIEDEMANN PE.Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents:verification of the concentration of chlorophyll standards by atomic absorption spectroscopy[J].Biochimica et Biophysica Acta Bioenergetics,1989,975:384-394.
[9]	BROUWER B,ZIOLKOWSKA A,BAGARD M, KEECH O,et al.The impact of light intensity on shade-induced leaf senescence[J].Plant cell and environment,2012,35:1084-1098.
[10]	ALTSCHUL SF,MADDEN TL,ZHANG J,et al.Gapped BLAST and PIBLASTS:A new gene ration of protein database search programs[J].Nucleic acids rsearche ,1997,25(17):3389-3402
[11]	DENG Y Y,LI J Q,WU S F,etal.Integrated nr database in protein annotation system and its localization[J].Computer engineering,2006,32(5):71-74.
[12]	APWEILER R,BAIROCH A,WU CH,etal.The universal protein knowledge base[J].Nucleic aids rsearchce,2004,32:D115-D119.
[13]	ASHBURNER M,BALL C A,BLAKE JA,et al.Gene ontology:tool for the unification of biology[J].Nature genetics,2000,25(1):25-29.
[14]	TATUSOV R L,GALPERIN M Y,NATALE D A.The COG database:A tool for genome scale analysis of protein functions and evolution[J].Nucleic aids research,2000,28(1):33-36.
[15]	KOONIN E V,FEDOROVA N D,JACKSON JD,et al.A comprehensive evolutionary classification of proteins encoded in complete eukaryotic genomes[J].Genomebiology,2004,5(2):R7.
[16]	FINN R D,BATEMAN A,CLEMENTS J,et al.Pfam:The protein families database[J].Nucleic aids research,2014,42:D222.
[17]	KANEHISA M,GOTO S,KAWASHIMa S,et al.The KEGG resource for deciphering the genome[J].Nucleic acids research,2004,32:D277-D280.
[18]	XIE C,MAO X,HUANG J,et al.KOBAS 2.0:A web server for annotation and identification of enriched pathways and diseases[J].Nucleic aids research,2011,39:W316-W322.
[19]	EDDY S R.Profile hidden Markov models[J].Bioinformatics,1998,14(9):755-763.
[20]	LiVAK KJ,SCHMITTGEN TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method[J].Methods,2001,25:402-408.
[21]	CALNEL M G R,THOMLEY J H M.Temperature and CO2 responses of leaf and canopy photosynthesis:A clarification using the non-rectangularHyperbola model of photosynthesis.[J].Annals of botany,1998,82:883-892.
[22]	MORALES M,ABADÍA A,ABADÍA J.Photoinhibition and photoprotection under nutrient deficiencies,drought and salinity[A].DEMMIG A B,ADAMS W W,MATTOO A K. Photoprotection,gene regulation,and environment[M].Berlin:Springer-Verlag,2008.
[23]	LU C M,ZHANG J H.Heat-induced multiple effects on PS II in wheat plants[J].J Plant Physiol,2000,156:259-265.
[24]	EFEOLU B,EKMEKÇI Y,ÇIÇEK N. Physiological responses of three maize cultivars to drought stress and recovery[J].J S Afr Bot,2009,75:34-42.
[25]	SCHRADER S M,WISE R R,WACHOLTZ W F,et,al.Thylakoid membrane responses to moderately high leaf temperature in Pima cotton[J].Plant Cell Environ,2004,27:725-735.
[26]	SHARKEY T D.Effects of moderate heat stress on photosynthesis:Importance of thylakoid reactions, rubisco deactivation, reactive oxygen species,and thermotolerance provided by isoprene[J].Plant Cell Environ,2005,28:269-277.
[27]	FERREIRA S,HJERNØ K,LARSEN M,et al.Proteome profiling of Populus euphratica Oliv. upon heat stress[J].Ann Bot,2006, 98:361-77.
[28]	MASCLAUX-DAUBRESSE C,REISDORF-CREN M,ORSEL M.Leaf nitrogen remobilisation for plant development and grain filling[J].Plant biology,2008,10,23-36.
[29]	Parry M A.Rubisco activity and regulation as targets for crop improvement[J].J Exp Bot,2013,64(3):717-730.

桑树光合作用转录组研究

Research on mulberry photosynthesis transcriptome

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 29

相关文章 12

编辑推荐

Metrics

本文评价

[1]	李喜莲, 郭建林, 李倩, 施伟达, 黄振远, 顾志敏. 红螯螯虾转录组中的SSR位点信息分析[J]. 湖北农业科学, 2020, 59(7): 207-211.
[2]	周燕, 余军, 张锐, 李倩, 李雪, 陈欣. 氮肥与烯效唑耦合对主干结果形核桃光合特性的影响[J]. 湖北农业科学, 2020, 59(4): 45-50.
[3]	王晓曼, 王琼珊, 王孝刚, 张教海, 张友昌, 张兴中, 别墅, 夏松波. 低温胁迫下两叶一心期棉花叶片的生理响应和转录组分析[J]. 湖北农业科学, 2020, 59(24): 169-176.
[4]	朱元芳, 徐永清, 陈芾葳, 冯艳忠, 刘娣, 李凤兰. EM菌生物有机肥对马铃薯脱毒苗生产微型薯的影响[J]. 湖北农业科学, 2020, 59(20): 46-50.
[5]	张景润, 王彬存. 春季温室内两种竹类植物叶片解剖结构与光合特性研究[J]. 湖北农业科学, 2020, 59(20): 108-111.
[6]	王瑞娴, 楚渠, 彭云武, 孟刚, 梁嘉俊, 周晓帆. 基于叶绿体matK基因序列的两份安康野生桑遗传特征分析[J]. 湖北农业科学, 2020, 59(15): 151-154.
[7]	岳高峰, 韩志强, 薛志伟, 刘辉, 杨文月. 遮阴处理对草莓光合特性和产量品质的影响[J]. 湖北农业科学, 2020, 59(11): 84-88.
[8]	金方伦, 王贤玉, 韩世玉, 罗朝斌, 杨胜特, 黎明, 胡世叶. 桑树叶片大小与质量的相关性[J]. 湖北农业科学, 2020, 59(1): 74-77.
[9]	周勇辉, 刘小平, 罗素梅, 赖金莉, 陶秀花. 两种彩叶桂花新品种光合特性的比较研究[J]. 湖北农业科学, 2019, 58(24): 124-127.
[10]	莫荣利, 秦光良, 解金林, 于翠, 李勇, 邓文, 胡兴明. 桑园测土配方优化施肥试验研究[J]. 湖北农业科学, 2019, 58(17): 37-40.
[11]	周建华, 李勇, 于翠, 莫荣利, 朱志贤, 董朝霞, 胡兴明, 邓文. 不同繁育方式对果桑光合特性和糖代谢的影响[J]. 湖北农业科学, 2019, 58(16): 89-95.
[12]	刘昭, 王丽娟, 杜梦卿, 连朋. 不同草莓品种主要性状差异研究[J]. 湖北农业科学, 2018, 57(16): 79-82.